Journal: Frontiers in Immunology
Article Title: Larval ascariasis elicits a prominent IgA and IgG1/2 antibody response to adult Ascaris excretory/secretory antigens in pigs
doi: 10.3389/fimmu.2025.1606128
Figure Lengend Snippet: Strong positive correlation between sIgA against A . suum adult ES and eosinophil influx. (A) Pearson correlation of sIgA against adult ES products and %eosinophil frequencies of leukocytes in BAL . (B) Box plots illustrating median IgM, IgG and IgA responses against A . suum adult ES products in BAL fluid. (C) Box plots illustrating sIgA against L3 lysate, adult lysate and adult ES in BAL fluid. Blue and red denotes uninfected controls and A. suum infected pigs, respectively. Whiskers indicate 95% percentile. Significance determined by Wilcoxon test is represented by p<0.05: *p<0.01: **.
Article Snippet: For IgM, IgA, IgG1 and IgG2, non-conjugated mouse anti-pig IgM (Bio-Rad, # MCA637GA) diluted at 1:20000, mouse anti-pig IgA (Bio-Rad, # MCA638GA) diluted at 1:1000, mouse anti-pig IgG1 (Bio-Rad, # MCA635GA) diluted at 1:1000 and mouse anti-pig IgG2 (Bio-Rad, # MCA638GA) diluted at 1:1000, mouse anti-pig sIgA (Bio-Rad, # MCA634GA) diluted at 1:10000 was used.
Techniques: Infection